Seed Biotechnology Center
Seed Biotechnology Center
Seed Biotechnology Center
University of California
Seed Biotechnology Center

Cotton

Development of a haploid induction system for cotton

Cotton
In collaboration with David Tricoli (UC Davis) and David Stelly (Texas A&M), the SBC is translating research developed in Arabidopsis in the Simon Chan lab to cotton. Chan identified a specific chromosomal protein (CenH3) that can be modified to simplify the development of haploid plants. To continue the work from 2012, the orthologous gene from cotton was isolated, modified and transformed into cotton at the Ralph M. Parsons Foundation Plant Transformation Facility (UC Davis). Transgenic plants were created in 2013 and are being evaluated for capacity to induce haploids. Furthermore, a more efficient transformation protocol for cotton is being developed. This work was funded by Cotton Incorporated.


Anchoring and characterizing variation in the cotton genome

In an effort to fully leverage cotton genomics for plant breeding, the SBC coordinated partnerships with David Stelly (Texas A & M) and BGI.  The genomes of 11 G. hirsutum lines (Upland) and one G. barbadense (Pima) line were sequenced to define DNA diversity in breeding materials. Unlike the limited diversity found in the genome (as revealed in transcriptomes), a wealth of DNA changes was detected in the non-coding regions. Led by Amanda Hulse (in Stelly’s group) and Hamid Ashrafi (UC Davis), a DNA Illumina array containing 70,000 markers was created and made available for breeders to analyze their germplasm. This collaboration included public and private institutions from Australia, India, France and United States.  This work was funded by Cotton Incorporated.

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