| Therapeutic Proteins | Plantibodies | Allergens | Vaccines | Other proteins |
| Protein Expression Systems | Plant Transformation systems |
| Viral Expression systems | Promoter and targeting systems |
| Production of Pharmaceutical Crops | Government oversight |
| Production practices | Biotech Companies |
Production of Therapeutic Proteins in Plants 8078
Introduction
Biotechnology Industry Organization: Approved Biotech Drugs, 1999
Pharmaceutical Research and Manufacturers of America
369 Biotechnology Medicines in Testing, Pharmaceutical Research and Manufacturers of America
BioSpace.com - Biotech, Biotechnology and Pharmaceutical News, Jobs, Companies, Stocks
Fields of Gene Factories, LA times 04june2001
The Manufacture of Medical and Health Products By Transgenic Plants. 2001.
by Esra Galun (The Weizmann Institute of Science, Israel) & Eithan
Galun (Hadassah University Hospital, Israel) http://www.icpress.co.uk/
Recombinant proteins from plants : production and isolation of clinically useful compounds /, edited by Charles Cunningham and Andrew J.R. Porter. Totowa, N.J. : Humana Press, c1998.
H Daniell et al. 2001. Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends in Plant Science 6, 219-226
Giddings, Glynis; Allison, Gordon; Brooks, Douglas; Carter, Adrian.Transgenic plants as factories for biopharmaceuticals. In: Nature Biotechnology November, 2000. 18 (11): 1151-1155.
EE Hood, JM Jilka. 1999. Plant-based production of xenogenic proteins. Current Opinion in Biotechnology 10, 382-386
Stefan, S., Emans, N., Fischer, R. 2002. Antibody molecular farming in plants and plant cells. Phytochemistry Reviews 1: 45-54.
Therapeutic proteins - Plantibodies
From 'Plantibodies' to Profits?, wired news 27june2001
Bio-Tech Info - Other Applications - Pharming
Phytomedics, Inc. - Biotechnology, biopharmaceutical, and nutraceutical company
McCormick, Alison A.; Kumagai, Monto H.; Hanley, Kathleen; Turpen,
Thomas H.; Hakim, Itzhak; Grill, Laurence K.; Tuse, Daniel; Levy, Shoshana;
Levy, Ronald.Rapid production of specific vaccines for lymphoma by
expression of the tumor-derived single-chain Fv epitopes in tobacco plants.
In: Proc Natl Acad Sci USA Jan. 19, 1999. 96 (2): 703-708.
Gavilondo, Jorge V.; Larrick, James W.. Antibody engineering at the millennium. In: Biotechniques July, 2000. 29 (1): 128-145.
Therapeutic proteins - Allergens
Breiteneder, H.; Krebitz, M.; Wiedermann, U.; Wagner, B.; Essl, D.; Steinkellner, H.; Turpen, T. H.; Ebner, C.; Buck, D.; Niggemann, B.; Scheiner, O..Rapid production of recombinant allergens in Nicotiana benthamiana and
their impact on diagnosis and therapy.
International Archives of Allergy and Immunology 2001. 124, 48-50.
Crameri, Reto; Kodzius, Rimantas; Konthur, Zoltan; Lehrach, Hans;
Blaser, Kurt; Walter, Gerald.Tapping allergen repertoires by advanced cloning technologies.
International Archives of Allergy and Immunology 2001. 124: 43-47.
Gadermaier, Gabriele; Krebitz, Monika; Wagner, Birgit; Breiteneder,
Heimo; Obermeyer, Gerhard; Ferreira, Fatima (57th Annual Meeting of the
American Academy of Allergy, Asthma and Immunology New Orleans, Louisiana, USA
March 16-21, 2001).Plant-based production of recombinant mugwort allergens.
Journal of Allergy and Clinical Immunology 2001. 107 (2): S56-S57.
Krebitz, Monika; Peterbauer, Clemens; Ferreira, Fatima; Essl,
Dagmar; Steinkellner, Herta; Scheiner, Otto; Breiteneder, Heimo (57th Annual
Meeting of the American Academy of Allergy, Asthma and Immunology New Orleans,
Louisiana, USA March 16-21, 2001).Production of Mal d 2, a major allergen
from apple (Malus domestica), in a plant based expression system, and its biological activity against pathogens.
Journal of Allergy and Clinical Immunology February, 2001. 107 (2): S188-S189.
Therapeutic proteins - Vaccines
Daniell Lab for Molecular Biotechnology Research
H Daniell et al. 2001. Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends in Plant Science 6, 219-226
Langridge, WHR. 2000. Edible vaccines. Scientific American 283 (3): 66-71
Kapusta, J.; Modelska, A.; Figlerowicz, M.; Pniewski, T.;
Letellier, M.; Lisowa, O.; Yusibov, V.; Koprowski, H.; Plucienniczak, A.;
Legocki, A. B..A plant-derived edible vaccine against hepatitis B virus.
In: FASEB Journal Oct., 1999. 13 (13): 1796-1799.
Tackaberry, E. S.; Dudani, A. K.; Prior, F.; Tocchi, M.; Sardana,
R.; Altosaar, I.; Ganz, P. R..Development of biopharmaceuticals in plant
expression systems: Cloning, expression and immunological reactivity of human
cytomegalovirus glycoprotein B (UL55) in seeds of transgenic tobacco. In:
In: Vaccine Aug. 6, 1999. 17 (23-24): 3020-3029.
Walmsley, Amanda M.; Arntzen, Charles J..Plants for delivery of edible vaccines.
In: Current Opinion in Biotechnology April, 2000. 11 (2): 126-129.
Shivprasad, Shailaja; Pogue, Gregory P.; Lewandowski, Dennis J.;
Hidalgo, Joann; Donson, Jonathan; Grill, Laurence K.; Dawson, William
O..Heterologous sequences greatly affect foreign gene expression in tobacco mosaic virus-based vectors.
In: Virology March 15, 1999. 255 (2): 312-323.
Della-Cioppa, Guy; Grill, Laurence K. (Conference Lexington, Kentucky,
USA October 1-4, 1995).Production of novel compounds in higher plants by
transfection with RNA viral vectors. Collins, G. B. Shepherd, R. J., In:
Annals of the New York Academy of Sciences; Engineering plants for commercial
products and applications. New York Academy of Sciences ; 2 East 63rd Street,
New York, New York 10021, USA, 1996. 57-61.
Production of recombinant antigens in plants for animal and human immunization - 1997
Therapeutic proteins - Other proteins
Genetic Engineering and the Allergy Issue, PLANT PHYSIOLOGY 126 (1): 5
Crumbs of comfort (hypoallergenic wheat), agbios 24may2001
A strategy for the identification of proteins targeted by thioredoxin, Yano et al., PNAS 98 (8): 4794
Yano, Hiroyuki; Wong, Joshua H.; Lee, Young Moo; Cho, Myeong-Je;
Buchanan, Bob B..A strategy for the identification of proteins targeted by thioredoxin.
In: Proc Natl Acad Sci USA April 10, 2001. 98 (8): 4794-4799.
H Daniell et al. 2001. Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends in Plant Science 6, 219-226
Suzuki, Y. A.; Kelleher, S. L.; Lonnerdal, B. Federation of American Societies for Experimental Biology. (Annual Meeting of Professional Research Scientists: Experimental Biology 2000 San Diego, California, USA
April 15-18, 2000).Thermal, pH, and digestion stability of recombinant human lactoferrin expressed in rice.
In: FASEB Journal March 15, 2000. 14 (4): A218.
Chong, DKX; Langridge, WHR.Expression of full-length bioactive antimicrobial
human lactoferrin in potato plants.
In: TRANSGENIC RESEARCH, FEB, 2000, V9(N1):71-78.
Chong, DKX; Roberts, W; Arakawa, T; Illes, K; Bagi, G; Slattery, CW;
Langridge, WHR.Expression of the human milk protein beta-casein in
transgenic potato plants.
TRANSGENIC RESEARCH, JUL, 1997, V6(N4):289-296.
Protein Expression Systems
Plant Transformation systems
Biotechnology Provides New Tools for Plant Breeding - Suslow, Thomas & Bradford - ABC Series
Electro-Transformation Technique Sparks Pharmable Plants, ASPB Press Releases
JM Staub et al. High-yield production of a human therapeutic protein in tobacco chloroplasts. Nature Biotechnology 18, 333-338
H Koprowski, V Yubisov. 2001. The green revolution: plants as heterologous expression vectors. Vaccine 19, 2735-2741
G Giddings, G Allison, D Brooks, A Carter. 2000. Transgenic plants as factories for biopharmaceuticals. Nature Biotechnology 18, 1151-1155
Koprowski, H.; Yusibov, V..The green revolution: Plants as heterologous expression vectors.
In: Vaccine 21 March, 2001. 19 (17-19): 2735-2741.
Komarnytsky, Slavko; Borisjuk, Nikolai V.; Borisjuk, Ljudmila G.; Alam, Muhammad Z.; Raskin, Ilya. Production of recombinant proteins in tobacco guttation fluid. In: Plant Physiology (Rockville) November, 2000. 124 (3): 927-933
Gleba, Doloressa; Borisjuk, Nikolai V.; Borisjuk, Ludmyla G.; Kneer, Ralf; Poulev, Alexander; Skarzhinskaya, Marina; Dushenkov, Slavik; Logendra, Sithes; Gleba, Yuri Y.; Raskin, Ilya. Use of plant roots for phytoremediation and molecular farming. In: Proc Natl Acad Sci USA May 25, 1999. 96 (11): 5973-5977.
JG Boothe et al. 1997. Molecular farming in plants: oilseeds as vehicles for the production of pharmaceutical proteins. Drug Development Research 42, 172-181
Promoter and targeting systems
Thomas BR, Chandler J, Simmons CR, Huang N, Karrer E, Rodriguez RL (1994) Gene regulation and protein secretion from plant cell cultures: the rice alpha-amylase system. In: Ryu DDY, Furusaki S (eds) Advances in Plant Biotechnology, Elsevier, Amsterdam, pp 37-55
Kloesgen, Ralf Bernd.Protein transport into and across the thylakoid membrane.
In: Journal of Photochemistry and Photobiology B Biology 1997. 38 (1): 1-9.
Choi, Kyu Ri; Roh, Kwang-Soo; Kim, Jeong-Kook; Sim,
Woong-Soep.Genomic cloning and characterization of mitochondrial elongation
factor Tu (EF-Tu) gene (tufM) from maize (Zea mays L.).
In: Gene (Amsterdam) 31 October, 2000. 257 (2): 233-242.
Chytilova, Eva; Macas, Jiri; Sliwinska, Elwira; Rafelski, Susanne
M.; Lambert, Georgina M.; Galbraith, David W..Nuclear dynamics in
Arabidopsis thaliana. In: Molecular Biology of the Cell August, 2000. 11 (8): 2733-2741.
(Movies of nuclear movement available online at http://www.molbiolcell.org )
Hwang Y-S, Thomas BR, Rodriguez RL (1999) Differential expression of rice alpha-amylase genes during seedling development under anoxia. Plant Mol Biol 40, 911-920
Hwang Y-S, Karrer EE, Thomas BR, Chen L, Beachy RN, Rodriguez RL (1997) Three cis-elements required for rice alpha-amylase Amy3D expression during sugar starvation. Plant Mol Biol 36, 311-341
Boetti, H.; Chevalier, L.; Denmat, L. A.; Thomas, D.; Thomasset,
B..Efficiency of physical (light) or chemical (ABA, tetracycline, CuSO4 or
2-CBSU)-stimulus-dependent gus gene expression in tobacco cell suspensions.
In: Biotechnology and Bioengineering July 5, 1999. 64 (1): 1-13.
Chemical-inducible systems for regulated expression of plant genes,
Jianru Zuo and Nam-Hai Chua
Curent Opinion in Biotechnology 11, 146-151 (2000)
Production of Pharmaceutical Crops
Government oversight
Animal and Plant Health Inspection Service APHIS USDA
Center for Biologics Evaluation & Research CBER FDA
Guidance Page CDER FDA
GMP Guide for Active Pharmaceutical Ingredients, FDA 31july2000
Plant-Derived Biologics Seminar and Public Hearing, Iowa St Univ 05april2000
Plant-Derived Biologics Seminar and Public Hearing PT 2, Iowa St Univ 06april2000
Case Studies of Environmental Regulation for Biotechnology, OSTP (see Case 3, pg 47)
KE Stein, KO Webber. 2001. The regulation of biologic products derived from bioengineered plants. Current Opinion in Biotechnology 12, 308-311
Production practices
Association of Official Seed Certifying Agencies
Testimony of the BIO Before the Maryland House of Delegates Environmental Matters Committee 06march2001
Terminator on trial , RAFI 12may2000
Destructive Consequences of "Controlling Plant Gene Expression" for Food Security and Biodiversity, TWN 02june1999
RAFI's annual update on Terminator and Traitor Technology, RAFI jan2001
Submission to the Royal Society of Canada's Expert panel on the future of food biotechnology, Conservation Council of Ontario 28april2000
Genetic Engineering Dictionary, Agroecology/Sustainable Agriculture Program (ASAP)
GMOs: Looking At Both Sides of the Controversy, Agroecology/Sustainable Agriculture Program (ASAP)
Good Agricultural Practices (GAP): Guide to Minimize Microbial Food Safety Hazards for Fresh Fruits and Vegetables, FDA/CFSAN Final Guidance for Industry 09march2000
Current Good Manufacturing Practice (CGMP) Regulations, CDER FDA
BioGMP Training Software, SPI USA Inc.
Biotech Companies
Applied Phytologics, Inc.
AltaGen Biosciences
Dow AgroSciences
Large Scale Biology (formerly Biosource)
Meristem Therapeutics
Phytomedics, Inc.
Researchers developing technology to help farmers "grow" pharmaceuticals
ProdiGene Inc
SemBioSys
Ventria Bioscience